脐血与老人外周血中TIMP2蛋白检测的影响因素及意义

目的探讨不同血浆处理方式对脐血和老人外周血血浆中TIMP2蛋白检测的影响，建立人血浆中TIMP2蛋白检测方法的同时为联合干细胞延缓衰老提供理论基础。 方法采集了15份脐带血和15份老人外周血，利用不同的离心力、不同的储存温度和不同的检测试剂盒对血浆中TIMP2蛋白最终检测含量的影响。多组均数差异比较采用单因素方差分析，两组比较采用独立样本t检验。 结果1?000×g、1?500×g和2?000×g离心力组组间TIMP2蛋白含量差异无统计学意义（P?> 0.05）。4 ℃、-20?℃、-80?℃保存组，保存一周或者两周TIMP2蛋白含量差异均无统计学意义（P?> 0.05）。利用R&D Systems、Abnova和Abcam检测试剂盒均不对全血中TIMP2蛋白最终检测量产生显著影响（P?>?0.05）。脐带血中的TIMP2蛋白含量（147.00±15.92）?pg/ml高于老人外周血中TIMP2蛋白含量（79.56±10.80）?pg/?ml，差异具有统计学意义（P?< 0.01）。 结论不同离心力、血浆储存温度及检测试剂盒间均不影响脐血和老人外周血中TIMP2蛋白最终的检测含量，且脐血血浆中TIMP2蛋白含量高于老人外周血。显示TIMP2蛋白可能与衰老存在直接的相关性，具有与干细胞联合治疗并延缓衰老的潜在价值。

Detection of TIMP2 protein in plasma between the cord blood and peripheral blood of the elder

ObjectiveTo explore the effects of different treatments on the detection of TIMP2 protein in umbilical cord blood or peripheral blood of elder. The establishment of a method for detecting the stability of TIMP2 protein in human plasma provided a theoretical basis for the possibility of combining stem cells to anti-aging. Methods15 umbilical cord blood samples and 15 peripheral blood samples of elder were collected. The effects of final detection of TIMP2 protein in plasma were investigated, which was used different centrifugal forces, different storage temperatures and different test kits. The difference between the two groups were compared by one-way ANOVA and independent sample t-test. ResultsThere was no significant difference in TIMP2 protein content among 1?000×g centrifugal force, 1?500×g centrifugal force and 2?000×g centrifugal force. There was no significant difference in TIMP2 protein content among 4℃, -20℃ and -80℃ storage temperature. The R&D Systems, Abnova and Abcam test kits did not significantly affect the final detection of TIMP2 protein in whole blood. Moreover, the contents of TIMP2 protein in umbilical cord blood (147.00±15.92) pg/ml were significantly higher than that in the peripheral blood of the elder (79.56±10.80, P?< 0.01) . ConclusionDifferent centrifugal forces, different storage temperatures and different test kits did not affect the final detection of TIMP2 in the cord blood and peripheral blood of the elder. Moreover, the contents of TIMP2 protein in the cord blood were significantly higher than that in the peripheral blood of the elder, which indicated that TIMP2 protein had a direct relationship of aging. It is of a potential value in anti-aging combined with the stem cells.