Three-dimensional antennal lobe atlas of the oriental fruit moth, <Emphasis Type="Italic">Cydia molesta</Emphasis> (Busck) (Lepidoptera: Tortricidae): comparison of male and female glomerular organization |
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Authors: | Nélia Varela Louise Couton César Gemeno Jesús Avilla Jean-Pierre Rospars Sylvia Anton |
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Institution: | (1) Department of Crop Protection Centre UdL-IRTA, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain;(2) INRA, UMR 1272 Physiologie de l’Insecte, 78000 Versailles, France;(3) Department of Crop and Forest Science, University of Lleida, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain;(4) INRA, UMR 1272, Route de St. Cyr, 78026 Versailles Cedex, France |
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Abstract: | The oriental fruit moth Cydia molesta is an important pest and the behavioural role of olfactory signals such as pheromones and plant volatiles have been studied
extensively in both sexes. To understand odour processing further, however, detailed knowledge of the anatomy of the olfactory
system is crucial. In the present study, an atlas of the antennal lobe (AL) is presented based on the three-dimensional reconstructions
of both ALs of three male and three female brains by means of neuroanatomical and computational approaches. We identified
48–49 "ordinary" glomeruli and one large glomerulus situated at the entrance of the antennal nerve in males, and 49–52 "ordinary"
glomeruli and one large glomerulus in the ventro-medial part of the AL in females. Anomalous supernumerary, anomalous missing
and sexually dimorphic glomeruli were found in the studied individuals in greater numbers than in other lepidopteran species.
Male and female maps were compared with respect to glomerular size and position with 45 glomeruli being matched, indicating
a conserved glomerular pattern between the sexes. Three additional glomeruli were sexually dimorphic in size and five male-specific
and six female-specific glomeruli were also found. Palp backfills resulted in the staining of a unique glomerulus in both
sexes identified as the sexually dimorphic glomerulus 45. This glomerulus was never stained from antennal backfills, which
stained the other glomeruli of the AL. The three-dimensional atlas can now be used to elucidate the functional role of individual
glomeruli in both sexes of C. molesta.
Nélia Varela and Louise Couton contributed equally to this work.
This study was supported by research grants from INRA (Projet Jeune Equipe and Projet S.P.E.) to S.A. and J.P.R. and by the
Spanish Ministry of Science and Technology (research grant AGL2004-05812/AGR to J.A. and C.G., Spanish Science and Education
Department, MEC). N.V. was financed by fellowship no. BES-2005-7605 (MEC, Spain). |
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Keywords: | Olfaction Computational neuroanatomy Atlas Antennal lobe Oriental fruit moth Cydia molesta (Insecta) |
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