Proteolytic activity among various oral Treponema species and cloning of a prtP-like gene of Treponema socranskii subsp. socranskii |
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| Authors: | Heuner K Bergmann I Heckenbach K Göbel U B |
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| Institution: | 1. Single-Cell Center, CAS Key Laboratory of Biofuels and Shandong Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China;2. Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong, China;3. University of Chinese Academy of Sciences, Beijing, China;1. Institute of Pharmacy, University of Tartu, Nooruse 1, 50411 Tartu, Estonia;2. Institute of Technology, University of Tartu, Nooruse 1, 50411 Tartu, Estonia |
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| Abstract: | The proteolytic activity of 11 treponemal strains representing different phylogenetic groups was investigated by SDS-polyacrylamide gel electrophoresis with copolymerised casein, gelatin and fibrinogen as substrates. The activity was specified to be trypsin- and chymotrypsin-like by the cleavage of synthetic substrates BAPNA and SAAPFNA, respectively. Nine strains degrade casein and the synthetic substrate BAPNA. Chymotrypsin-like activity specifically inhibited by phenylmethylsulfonyl fluoride was found in four treponemes. Southern blot analysis using a Treponema socranskii subsp. socranskii-specific prtP probe confirmed the presence of prtP homologous genes in these four strains. The internal fragments of the chymotrypsin-like protease genes were cloned and sequenced after PCR amplification. Here we report the cloning of the complete prtP-like gene of T. socranskii subsp. socranskii, an organism shown to possess epidemiologic relevance in periodontitis. |
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