| 伪狂犬病毒gE基因在昆虫细胞中的高效表达 |
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| 引用本文： | 方六荣,陈焕春,等.伪狂犬病毒gE基因在昆虫细胞中的高效表达[J].生物工程学报,2001,17(4):449-451. |
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| 作者姓名： | 方六荣 陈焕春 |
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| 作者单位： | 方六荣（华中农业大学牧医学院动物病毒室,武汉,430070）
陈焕春（华中农业大学牧医学院动物病毒室,武汉,430070）
肖少波（华中农业大学牧医学院动物病毒室,武汉,430070）
何启盖（华中农业大学牧医学院动物病毒室,武汉,430070）
王革非（华中农业大学牧医学院动物病毒室,武汉,430070） |
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| 基金项目： | 国家重点科技项目(攻关)计划(96-C01-04-03). |
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| 摘 要： |
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| 关 键 词： | 伪狂犬病毒 gE基因 昆虫细胞 表达 乳胶凝集试验 动物病毒 |
| 文章编号： | 1000-3061(2001)04-0449-03 |
| 修稿时间： | 2001年1月20日 |
Expression of the gE Gene of Pseudorabies Virus In Insect Cells |
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| L R Fang,H C Chen,S B Xiao,Q G He,G F Wang.Expression of the gE Gene of Pseudorabies Virus In Insect Cells[J].Chinese Journal of Biotechnology,2001,17(4):449-451. |
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| Authors: | L R Fang H C Chen S B Xiao Q G He G F Wang |
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| Institution: | Laboratory of Animal Virology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. |
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| Abstract: | In order to develop a simple and safe test for the detection of vaccinated as well as wild type Pseudorabies virus (PRV) infected pigs, the modified gE gene of PRV Ea strain, obtained by cutting the 5' UTR using PCR and DNA recombinant technique, was inserted into baculovirus expression vector pFastBac 1, resulting the trans-position plamid pFE1.75. After homologous recombination, recombinant baculovirus rvBacE1.75 was gained and high level expression of glycoprotein E (gE) was observed after the infection of rvBacE1.75 to Tn-5B1-4 cells. The expression product was 80-88 kD and was specific to antisera against PRV Ea strain by Western-blotting. Purified recombinant proteins were used as an antigen in Latex Agglutination Test(gE-LAT) and the test was specific, sensitive, safe and simple. |
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| Keywords: | pseudorabies virus(PRV) gE gene insect cells expression latex agglutination test (LAT) |
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