Over expression of PQQ glucose dehydrogenase in Escherichia coli under holo enzyme forming condition期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Over expression of PQQ glucose dehydrogenase in Escherichia coli under holo enzyme forming condition

Authors:	Koji Sode Arief Budi Witarto Kazumoto Watanabe Keisuke Noda Shunsuke Ito Wakako Tsugawa

Affiliation:	(1) Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei-shi, 184 Tokyo, Japan

Abstract:	Summary The over expression of PQQ glucose dehydrogenase PQQGDH was investigated. The level of PQQGDH expressed in E.coli PP2418/pGEc1 was more than 10 fold when the cultivation was carried out under holo enzyme forming condition in the presence of 600 nM of PQQ and 10 mM of MgCl₂, compared with those of apo condition. It may be due to the difference in thermal stability of apo and holo PQQGDH.

Keywords:	PQQ glucose dehydrogenase over expression Esherichia coli thermal stability
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