Characterization of hCG regulation in cultured human amniotic fluid cells |
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Authors: | Caroline H Laundon Jean H Priest Robert E Priest |
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Institution: | (1) Department of Pathology and Laboratory Medicine, Division of Medical Genetics, Emory University, 30322 Atlanta, Georgia;(2) Department of Pediatrics, Division of Medical Genetics, Emory University, 30322 Atlanta, Georgia |
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Abstract: | Summary We showed previously that sodium butyrate stimulated human chorionic gonadotropin (hCG) measured by radioimmunoassay of medium
from human second trimester amniotic fluid cell cultures, termed AF cells. We now find that stimulation of hCG in the presence
of sodium butyrate takes as long as 20 h. When AF cells are preincubated with sodium butyrate, hCG levels increase in direct
relation to length of the preincubation period. These findings suggest that elevation of hCG is not due merely to a release
of hormone from the cells. Addition of cycloheximide or Actinomycin D inhibited protein synthesis and RNA synthesis, respectively,
and prevented the stimulation of hCG by sodium butyrate. These results lend support for a mechanism of regulation involving
protein and RNA synthesis, the increase in hCG levels being due to new synthesis of the hormone.
Other agents reported to influence hCG production by different types of cell cultures include dibutyryl cyclic AMP, epidermal
growth factor (EGF), methotrexate, and hydroxyurea. Dibutyryl cyclic AMP and EGF have no effect on hCG production in our AF
cells: methotrexate causes a minimal increase, hydroxyurea causes a further increase, but sodium butyrate has the strongest
stimulatory effect.
We conclude that amniotic fluid cells in culture are susceptible to environmental agents capable of modulating synthesis of
hCG by mechanisms involving synthesis of RNA and protein.
Research supported by Grant HD 11379 from the National Institutes of Health. |
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Keywords: | human chorionic gonadotropin sodium butyrate amniotic fluid cells |
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