| 11G工程细胞株人尿激酶原基因拷贝数的测定 |
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| 引用本文： | 张劲风,韩素文,俞炜源,胡宝成. 11G工程细胞株人尿激酶原基因拷贝数的测定[J]. 生物技术通讯, 1998, 0(1) |
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| 作者姓名： | 张劲风 韩素文 俞炜源 胡宝成 |
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| 作者单位： | 解放军大连医学高等专科学校，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所 进修生，北京 100071，北京 100071，北京 100071 |
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| 摘 要： | 采用DNA印迹和狭线印迹(Slot blot)的方法,对高效表达人尿激酶原(Pro-UK)的工程细胞11G含有的pro-UK基因拷贝数进行了测定。结果显示,11G工程细胞株内所含的pro-UK拷贝数为100～200/细胞。结果证明,11G细胞株是稳定高表达pro-UK的工程细胞,符合WHO规定的关于用于基因工程产品的外源基因转化细胞的标准。
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| 关 键 词： | 尿激酶原 拷贝数 工程细胞株 杂交 |
Detection of copy numbers of pro-UK gene in 11G cell line |
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| Zhang Jingfeng,Han Suwen,Yu Weiyuan,Hu Baocheng. Detection of copy numbers of pro-UK gene in 11G cell line[J]. Letters in Biotechnology, 1998, 0(1) |
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| Authors: | Zhang Jingfeng Han Suwen Yu Weiyuan Hu Baocheng |
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| Abstract: | Southern blot and Slot blot hybridization methods were used in this research to detect the copy numbers of pro-urokinase (pro-UK) gene in pro-UK-expression engineering cell line (11G cell line). The results showed that the copy number of pro-UK gene was about 200-300 copies/cell. It is demonstrated that pro-UK gene was amplificated by the cell culture with increasing concentrations of MTX in 11G engineering cell line. |
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| Keywords: | pro-urokinase copy number engineering cell line hybridization |
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