The Tumorgenicity of Glioblastoma Cell Line U87MG Decreased During Serial In Vitro Passage |
| |
Authors: | Yu Zeng Xizhao Wang Jizhou Wang Renhui Yi Hao Long Mingfeng Zhou Qisheng Luo Zhihao Zhai Ye Song Songtao Qi |
| |
Affiliation: | 1.Department of Neurosurgery, Nanfang Hospital,Southern Medical University,Guangzhou,People’s Republic of China;2.Department of Neurosurgery, The Fifth Affiliated Hospital,Sun Yat-Sen University,Zhuhai,People’s Republic of China;3.Gannan Medical University,Ganzhou,People’s Republic of China;4.Department of Neurosurgery,The First Affiliated Hospital of Gannan Medical University,Ganzhou,People’s Republic of China;5.Department of Neurosurgery,Affiliated Hospital of Youjiang Medical College for Nationalities,Baise,People’s Republic of China;6.Department of Neurosurgery,The Eighth Affiliated Hospital, Sun Yat-Sen University,Shenzhen,People’s Republic of China |
| |
Abstract: | Established cancer cell lines are routinely used to study cancer. Several factors such as serial passage may affect the reproducibility of experiments with cancer cell lines, but few researches focused on these changes. In the present study, different morphology and decreased tumorigenicity were observed in late passage U87MG cells. In vitro experiments further revealed that late passage U87MG cells possessed lower invasion properties than early passage, whereas no significant differences of proliferation and migration were found between early and late passage U87MG cells. In particular, we confirmed that late passage U87MG cells exhibited more epithelial phenotype with decreased PI3K/Akt pathway and TGF-β pathway expressions at protein level. In summary, our results focused on the changes of U87MG cells during serial in vitro passage, suggested that passage-induced changes may lead to notable changes of biological characteristics and several molecular transitions in cancer cell lines, indicating the necessity to shorten experiment-span and accomplish experiments with the same or similar passage cancer cell strains. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|