Production of poly(l-lactide)-degrading enzyme by Amycolatopsis orientalis for biological recycling of poly(l-lactide) |
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Authors: | Amnat Jarerat Yutaka Tokiwa Hideo Tanaka |
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Affiliation: | (1) CPR Co., Ltd., 4-102, Higashinobusue, Himeji Hyogo, 670-0965, Japan;(2) National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Higashi, Tsukuba Ibaraki, 305-8566, Japan;(3) Institute of Applied Biochemistry, University of Tsukuba, Tsukuba Ibaraki, 305-8572, Japan;(4) Present address: Food Technology Program, Mahidol University, Kanchanaburi Campus, Saiyok, Kanchanaburi, 71150, Thailand |
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Abstract: | Efficient production of poly(l-lactide)(PLA)-degrading enzyme was achieved by addition of 0.1% (w/v) silk fibroin powder into a liquid culture medium of an actinomycete, Amycolatopsis orientalis, without other complex nitrogen sources, such as yeast extract and peptone. Scaled-up production of the enzyme in a 5-l jar fermenter showed the possibility of producing this enzyme on an industrial scale at low production cost. The extracellular PLA-degrading enzyme showed potent degrading activity, which is effective for biological recycling of PLA, i.e., 2,000 mg/l of PLA powder was completely degraded within 8 h at 40°C using 20 mg/l purified enzyme. An optically active l-lactic acid with 600 mg/l was obtained as degradation product of PLA without undesirable racemization. |
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