Overexpression of the OsPDCD5 Gene Induces Programmed Cell Death in Rice |
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Authors: | Kotb ATTIA Ke-Gui LI Chun WEI Guang-Ming HE Wei SU Jin-Shui YANG |
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Affiliation: | Institute of Genetics, School of Life Sciences, Fudan University;, Shanghai 200433, China |
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Abstract: | The primary aim of the present study was to investigate the overexpression of the rice (Oryza sativa L.) programmed cell death 5 (OsPDCD5) gene in rice plant. Constitutive expression of OsPDCD5 from the cauliflower mosaic virus (CaMV) 35S promoter induced programmed cell death (PCD) in transgenic rice.Programmed cell death was accompanied by typical features, including inhibition of developmental growth,a reduction of fresh weight, degradation of total protein content, and fragmentation of genomic DNA. These results suggest that OsPDCD5 plays an essential role in the regulation of PCD in rice plants. |
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Keywords: | gene transformation Oryza sativa rice programmed cell death 5 (OsPDCD5) gene programmed cell death |
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