Microscope laser light scattering spectroscopy of single biological cells |
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| Authors: | I. Nishio J. Peetermans T. Tanaka |
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| Affiliation: | (1) Center for Polymer Studies, Department of Physics, Boston University, 02215 Boston, MA;(2) Department of Physics and Center for Materials Science and Engineering, Massachusetts Institute of Technology, 02139 Cambridge, MA |
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| Abstract: | A microscope laser light scattering setup was developed, allowing us to do intensity autocorrelation spectroscopy on the light scattered from a volume as small as (2 μm)3. This non-invasive technique makes cytoplasmic studies possible inside single live biological cells. The effect of osmotic swelling and shrinking on the diffusion coefficient of hemoglobin inside intact red blood cells is shown as an illustrative example of the applicability and sensitivity of this new experimental method. |
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| Keywords: | Microscope laser light scattering spectroscopy, of cells laser light scattering spectroscopy, of cells light scattering spectroscopy, of cells by laser microscopy spectroscopy of cells, microscope laser light scattering cells, laser light scattering spectroscopy of |
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