Identification ofporphyra lines (rhodophyta) by AFLP DNA fingerprinting and molecular markers |
| |
Authors: | Jian Wei Sun Min De Jin Chun Jiang Zhou Qing Kai Yang Man Li Weng Lin De Duan Pu Xu Jia Hai Ma Bin Wang |
| |
Affiliation: | (1) Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101 Beijing, P.R. China;(2) College of Life Science, Laiyang Agricultural University, 266109 Qingdao, P.R. China;(3) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, P.R. China;(4) Jiangsu Institute of Oceanology and Hydrology, 226007 Nantong, P.R. China;(5) Shanghai Fisheries University, 200090 Shanghai, P.R. China |
| |
Abstract: | Twenty-sevenPorphyra lines from 5 classes, including lines widely used in China, wild lines, and lines introduced to China from abroad in recent years, were screened by means of amplified fragment length polymorphism (AFLP) with 24 primer pairs. From the generated AFLP products, 13 bands that showed stable and repeatable AFLP patterns amplified by primer pairs M-CGA/E-AA and M-CGA/E-TA were scored and used to develop the DNA fingerprints of the 27Porphyra lines. Moreover, the DNA fingerprinting patterns were converted into computer language expressed with digitals 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of the corresponding band. On the basis of these results, computerized AFLP DNA fingerprints were constructed in which each of the 27Porphyra lines has its unique AFLP fingerprinting pattern and can be easily distinguished from others. Software called PGI-AFLP (Porphyra germ-plasm identification-AFLP) was designed for identification of the 27Porphyra lines. In addition, 21 specific AFLP markers from 15Porphyra lines were identified; 6 AFLP markers from 4Porphyra lines were sequenced, and 2 of them were successfully converted into SCAR (sequence characterized amplification region) markers. The developed AFLP DNA fingerprinting and specific molecular markers provide useful ways for the identification, classification, and resource protection of thePorphyra lines. |
| |
Keywords: | amplified fragment length polymorphism DNA fingerprinting Porphyra line sequence characterized amplified region sequence tagged site (STS) specific molecular marker |
本文献已被 SpringerLink 等数据库收录! |
|