Formation of leukotrienes and hydroxy acids by human neutrophils and platelets exposed to monosodium urate |
| |
Authors: | Charles N Serhan Ulf Lundberg Gerald Weissman Bengt Samuelsson |
| |
Institution: | 1. Dept. of Physiological Chemistry, Karolinska Institutet, S-104 01 Stockholm, Sweden;1. Division of Rheumatology, Dept. of Medicine, New York New York 10016, USA |
| |
Abstract: | Monosodium urate (MSU) crystals stimulate the production of arachidonic acid metabolites by human neutrophils and platelets. Neutrophils exposed to MSU generated leukotriene B4(LTB4). 6-
-LTB4, 12-
-6-
-LTB4, and 5S, 12S DHETE from endogenous sources of arachidonate. In addition to these metabolites both monohyroxyeicosatetraenoic acids (i.e., 5-HETE) and w-oxidation products (i.e., 20-COOH LTB4) were formed by neutrophils exposed to MSU. Addition of exogenous arachidonic acid led to increased formation of each of these metabolites. When neutrophils were treated with colchicine (10 uM), LTB4 but
5-HETE formation was impaired. (1-14C) Arachidonate-labeled platelets exposed to MSU released (1-14C)-arachidonate. (14C)-12 HETE, (14C)-HHT and (14C)-thromboxane B2. Results indicate that MSU stimulates arachidonic acid metabolism in both human neutrophils and platelets. Moreover, they suggest not only that metabolites of arachidonate may be considered as possible candidates for mediators of inflammation in crystal-associated diseases, but that colchicine blocks the formation of LTB4. |
| |
Keywords: | Leukotrienes monosodium urate crystals leukocytes platelets arachidonic acid colchicine |
本文献已被 ScienceDirect 等数据库收录! |
|