Orientation of Pea Stem Mitochondrial H+-Pyrophosphatase and Its Different Characteristics from the Tonoplast Counterpart |
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| Authors: | Vianello Angelo; Zancani Marco; Casolo Valentino; Macri Francesco |
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| Institution: | Dipartimento di Biologia ed Economia Agro-industriale, Sezione di Biologia Vegetale, Universitáa di Udine Via Cotonificio, 108, I-33100 Udine, Italy |
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| Abstract: | Proton pumping pyrophosphatase (H+-PPiase) of pea stem mitochondriaappears to be localized on the inner surface of the inner membrane.Aminohexanediphosphonate and dichloromethylenediphosphonateexert different inhibitory effects on this activity and on thatof tonoplast. Antibody raised against membrane-bound mitochondrialH+-PPiase does not react with tonoplast vesicles. Thus, plantmitochondrial H+PPiase seems to have a molecular structure differentfrom that of vacuolar H+-PPiase. (Received August 2, 1996; Accepted October 18, 1996) |
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