Conversion ofL-sorbose to 2-keto-L-gulonic acid by mixtures of immobilized cells ofGluconobacter melanogenus IFO 3293 andPseudomonas species |
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| Authors: | C. K. A. Martin D. Perlman |
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| Affiliation: | (1) School of Pharmacy, University of Wisconsin, Madison, Wisconsin, USA;(2) Present address: Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300 Braunschweig-Stöckheim, Germany |
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| Abstract: | Summary L-Sorbose was converted to 2-keto-L-gulonic acid by mixtures ofGluconobacter melanogenus IFO 3293 andPseudomonas syringae NRRL B-865 entrapped simultaneously in polyacrylamide gel. Since the temperature optima of both enzymatic reactions involved differed, a two-stage process with cells immobilized separately seems to offer a more efficient method to prepare 2-keto-L-gulonic acid.For preceeding paper in this series see Martin & Perlman (1976). |
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