Mass spectrometric measurements of the apolipoproteins of bovine (Bos taurus) HDL |
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| Authors: | Della Donna Lorenza Bassilian Sara Souda Puneet Nebbia Carlo Whitelegge Julian P Puppione Donald L |
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| Affiliation: | a Department of Animal Pathology, Division of Pharmacology and Toxicology, University of Turin, Italyb The Molecular Biology Institute, University of California, Los Angeles, CA 90095, USAc The Pasarow Mass Spectrometry Laboratory, The Jane & Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA |
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| Abstract: | As is the case in most mammals, high density lipoproteins (HDL) also comprise the major group of lipid carriers that circulate in bovine (Bos taurus) blood. As a continuation of our proteogenomic studies of mammalian apolipoproteins, we have obtained molecular masses for several of the apolipoproteins associated with bovine HDL. The major apolipoprotein on the HDL surface is apoA-I, but other apolipoproteins were also detected. Using electrospray-ionization mass spectrometry (ESI-MS), we report on values for apolipoproteins, A-I, proA-I and A-II, as well as post-translationally modified apoA-I. Analyses of tryptic fragments did reveal the presence of apoA-IV and apoC-III. However, in contrast to our previous studies of other mammalian HDL, we did not detect apoC-I. Interestingly, examination of the current assembly for the bovine genome does not show any evidence for an apoC-I gene. |
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| Keywords: | Mass spectrometry apoHDL apoC-I Cholesteryl ester transfer protein |
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