Polymorphic microsatellite loci for the Puerto Rican crested anole (<Emphasis Type="Italic">Anolis cristatellus</Emphasis>) and their amplification in related Puerto Rican species |
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Authors: | Richard E Glor Michele A Johnson Allan Larson |
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Institution: | (1) Center for Population Biology, University of California, Davis, CA 95616, USA;(2) Department of Biology, Washington University, St. Louis, MO 63130, USA;(3) Present address: Department of Biology, University of Rochester, RC Box 270211, Rochester, NY 14627, USA |
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Abstract: | We isolate six highly polymorphic microsatellite loci for the Puerto Rican crested anole (Anolis cristatellus) from a genomic library enriched for CA repeats. The number of alleles per locus ranged from 14 to 19, with levels of observed
heterozygosity ranging from 0.60 to 0.73. Most of these loci were successfully cross-amplified in other members of the cristatellus species group (A. evermanni, A. gundlachi, A. krugi, A. stratulus), but levels of polymorphism were lower. |
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Keywords: | Anolis lizards Microsatellite Puerto Rico |
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