Flow cytometric analysis of total protein content and size distributions of recombinantSaccharomyces cerevisiae |
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| Authors: | M. S. Peterson A. Y. Patkar J. -H. Seo |
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| Affiliation: | (1) School of Chemical Engineering, Purdue University, 47907 W. Lafayette, IN;(2) Department of Food Science and Technology, Seoul National University, 441-744 Suwon, S. Korea |
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| Abstract: | The total protein content and cell size distribution of recombinantSaccharomyces cerevisiae cells were analyzed by flow cytometry. The recombinant strain containing a regulatableSUC2 promoter and the host strain were compared when grown under similar conditions in a batch culture. Recombinant and host cells maintained similar size and total protein content while cloned-gene expression was repressed by glucose levels greater than 0.2% (w/v). Following derepression, recombinant cells demonstrated a mean total protein content and mean cell size 1.5–2 times greater than that of the host cells. In addition, these simple flow cytometric measurements of the changes in cell size and total protein content were found to closely follow diauxic growth ofSaccharomyces cerevisiae in batch culture. |
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