Comparison of wild type and genetically engineered nuclear polyhedrosis viruses of Autographa californica for mortality, virus replication and polyhedra production in Trichoplusia ni larvae |
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| Authors: | Yasuhisa Kunimi James R. Fuxa Bruce D. Hammock |
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| Affiliation: | (1) Department of Entomology, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, 70803 Baton Rouge, LA, USA;(2) Department of Entomology and Environmental Toxicology, University of California, 95616 Davis, CA, USA;(3) Present address: Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 183 Fuchu, Tokyo, Japan |
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| Abstract: | Virus replication and polyhedra production of two polyhedron-positive recombinant nuclear polyhedrosis viruses of Autographa californica, AcJHE.KK and AcAaIT which encode juvenile hormone esterase and scorpion toxin, respectively, were compared with those of a plaque purified wild-type nuclear polyhedrosis virus, AcMNPV-C6, in Trichoplusia ni larvae. Though average times required to kill the T. ni larvae increased with the age of the larvae, killing time by either recombinant virus was significantly shorter than that by wild-type virus. Killing time was reduced ca. 30% for AcAaIT-infected larvae and 5 to 8% for AcJHE.KK-infected larvae as compared to that for AcMNPV-C6-infected larvae. The average weight of larvae infected with AcAaIT was significantly lower than that of larvae infected with AcJHE.KK and AcMNPV-C6. The mean numbers of polyhedra produced in each larva inoculated with AcAaIT and AcJHE.KK were ca. 20% and 60%, respectively, of those for AcMNPV-C6. Total virus titers in AcMNPV-C6-infected larvae were significantly higher than those in AcJHE.KK- and AcAaIT-infected larvae until 2 days post infection. |
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| Keywords: | Trichoplusia ni recombinant nuclear polyhedrosis virus baculovirus virus replication polyhedra production |
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