Assessment of the efficiency of cotransformation of the T-DNA of disarmed binary vectors derived from Agrobacterium tumefaciens and the T-DNA of A. rhizogenes |
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Authors: | John D. Hamill Andrea Prescott Cathie Martin |
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Affiliation: | (1) Dept. of Genetics and Microbiology, Institute of Food Research (Norwich Laboratory), Colney Lane, NR4 7UA Norwich, UK;(2) Dept. of Genetics, John Innes Institute, Colney Lane, NR4 7UH Norwich, UK |
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Abstract: | Co-transfer of Agrobacterium rhizogenes T-DNA and T-DNA from the A. tumefaciens binary vector pBin19 (Bevan, 1984) was studied in detail using Nicotiana rustica. High frequencies of co-transfer of T-DNA's were observed, even when no selection pressure was exerted. Increased levels of pBin19 T-DNA were found in hairy root cultures with selection at higher levels of kanamycin sulphate (50–200 g ml–1). Several other species were also transformed by A. rhizogenes carrying pBin19 and A. rhizogenes harbouring a different binary factor, pAGS125 (Van den Elzen et al., 1985), was used to transform N. rustica hairy roots to confer hygromycin B resistance. |
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Keywords: | Agrobacterium rhizogenes binary vector kanamycin resistance transformation secondary metabolism |
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