| 酵母双杂交筛选类固醇激素合成急性调节蛋白的相互作用蛋白质 |
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| 引用本文： | 杨晓扬,于淼,王建,唐刘君,陈慧,金超智,张卜兮,詹轶群,王晓辉,杨晓明. 酵母双杂交筛选类固醇激素合成急性调节蛋白的相互作用蛋白质[J]. 生物技术通讯, 2012, 23(1): 6-10 |
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| 作者姓名： | 杨晓扬 于淼 王建 唐刘君 陈慧 金超智 张卜兮 詹轶群 王晓辉 杨晓明 |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所,蛋白质组学国家重点实验室,北京100850 |
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| 基金项目： | 国家自然科学基金（30371596）; 国家重点基础研究发展计划（2002CB513100） |
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| 摘 要： | 目的:利用酵母双杂交技术筛选人肝cDNA文库中与类固醇激素合成急性调节蛋白(STAR)相互作用的蛋白质。方法:将全长STAR基因克隆到酵母表达载体pDBLeu中,形成诱饵;将构建好的诱饵质粒转化至AH109酵母感受态中,利用酵母双杂交技术筛选人肝cDNA文库中与其相互作用的蛋白质,并进行相互作用的回转验证。结果:构建了酵母诱饵蛋白表达质粒pDBLeu-STAR,并筛选到6个猎物,其中有5对相互作用回转验证阳性。结论:为进一步研究STAR的功能和作用机制提供了新的线索。
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| 关 键 词： | 酵母双杂交 蛋白质相互作用 类固醇激素合成急性调节蛋白 |
Screening of the Proteins Interacting with Steroidogenic Acute Regulatory Protein via a Yeast Two-Hybrid System |
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| YANG Xiao-Yang , YU Miao , WANG Jian , TANG Liu-Jun , CHEN Hui , JIN Chao-Zhi , ZHANG Bu-Xi , ZHAN Yi-Qun , WANG Xiao-Hui , YANG Xiao-Ming. Screening of the Proteins Interacting with Steroidogenic Acute Regulatory Protein via a Yeast Two-Hybrid System[J]. Letters in Biotechnology, 2012, 23(1): 6-10 |
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| Authors: | YANG Xiao-Yang YU Miao WANG Jian TANG Liu-Jun CHEN Hui JIN Chao-Zhi ZHANG Bu-Xi ZHAN Yi-Qun WANG Xiao-Hui YANG Xiao-Ming |
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| Affiliation: | State Key Laboratory of Proteomics,Beijing Institute of Radiation Medicine,Beijing 100850,China |
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| Abstract: | Objective： To screen proteins interacting with steroidogenic acute regulatory protein（STAR） via a yeast two-hybrid system.Methods： The full length gene of STAR was cloned into the pDBLeu vector as the bait plasmid.The plasmid was transformed into yeast cell AH109.Then,yeast two-hybrid assay was used to screen the proteins that can interact with STAR from the human liver cDNA library.Yeast two-hybrid re-transformation assay was used to confirm the interactions.Results： The full length STAR gene was successfully cloned into pDBLeu vector.Six preys were obtained via a yeast two-hybrid screening,and five of them were confirmed by yeast two-hybrid re-transformation assay.Conclusion： The results provide new clues for further research of STAR. |
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| Keywords: | yeast two-hybrid protein-protein interaction steroidogenic acute regulatory protein |
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