MDCK单克隆细胞库的建立及检定

目的:建立甲型H1N1流感病毒疫苗株高适应MDCK单克隆细胞库,用于培养生产流感病毒疫苗,为细胞代替鸡胚制备流感病毒疫苗提供保证。方法:用有限稀释法将MDCK细胞进行单克隆化,通过血凝和TCID50筛选甲型H1N1流感病毒疫苗株的高适应性单克隆化细胞株,扩大培养建立细胞库并进行检定。结果:共制备了97株单克隆化MDCK细胞,筛选到甲型H1N1流感病毒疫苗株高适应性MDCK单克隆细胞株,扩大培养建立了细胞库,经检定符合《中华人民共和国药典.三部》(2010版)对细胞库的要求。结论:建立了甲型H1N1流感病毒疫苗株高适应性MDCK单克隆细胞库,为细胞培养生产流感病毒疫苗奠定了基础。

Establishment and Detection of HIN1 Influenza Virus Adapted Monoclonal MDCK Cell Bank

Objective： To search the H1N1 influenza virus adopted MDCK monoclonal cells and establish mono clonal cell bank, which could promote the production of the influenza virus in cells. Methods： The monoclonal cells of the MDCK cell acquired from ATCC were established by limited dilution and used to search the H1N1 in fluenza virus adopted monoelonal cells. The M87 monoclonal cell was filtered, and the mast cell bank and the work cell bank were established subsequently. Results： According to the Pharmacopoeia of P.R.China（2010, Part 3）, the cell banks were tested for identification, asepsis, mycoplasma, exogenous and endogenous viral factors. The detection assays indicated that the cell banks established was accorded with the rules. Conclusion： The cell banks could be used as substrate to produce the influenza virus vaccines.