The differentiation of human placenta-derived mesenchymal stem cells into dopaminergic cells in vitro |
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Authors: | Li Chen Dong-Mei He Yuan Zhang |
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Institution: | (1) Institute of Hematology, Medical College of Jinan University, Guangzhou, 510632, P. R. China |
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Abstract: | Mesenchymal stem cells (MSCs) constitute an interesting cellular source to promote brain regeneration after Parkinson’s disease.
MSCs have significant advantages over other stem cell types, and greater potential for immediate clinical application. The
aim of this study was to investigate whether MSCs from the human placenta could be induced to differentiate into dopaminergic
cells. MSCs from the human placenta were isolated by digestion and density gradient fractionation, and their cell surface
glycoproteins were analyzed by flow cytometry. These MSCs were cultured under conditions promoting differetiation into adipocytes
and osteoblasts. Using a cocktail that includes basic fibroblast growth factor (bFGF), all trans retinoic acid (RA), ascorbic
acid (AA) and 3-isobutyl-1-methylxanthine (IBMX), the MSCs were induced in vitro to become dopamine (DA) neurons. Then, the expression of the mRNA for the Nestin and tyrosine hydroxylase (TH) genes was
assayed via RT-PCR. The expression of the Nestin, dopamine transporter (DAT), neuronal nuclear protein (NeuN) and TH proteins
was determined via immunofluorescence. The synthesized and secreted DA was determined via ELISA. We found that MSCs from the
human placenta exhibited a fibroblastoid morphology. Flow cytometric analyses showed that the MSCs were positive for CD44
and CD29, and negative for CD34, CD45, CD106 and HLA-DR. Moreover, they could be induced into adipocytes and osteocytes. When
the MSCs were induced with bFGF, RA, AA and IBMX, they showed a change in morphology to that of neuronal-like cells. The induced
cells expressed Nestin and TH mRNA, and the Nestin, DAT, NeuN and TH proteins, and synthesized and secreted DA. Our results
suggest that MSCs from the human placenta have the ability to differentiate into dopaminergic cells. |
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Keywords: | Mesenchymal stem cell Human Placenta Differentiation Dopamine |
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