Structural analysis of heavy ion radiation-induced chromosome aberrations by atomic force microscopy |
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Authors: | Murakami M Minamihisamatsu M Sato K Hayata I |
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Institution: | Division of Radiobiology and Biodosimetry, National Institute of Radiological Sciences, 9-1, Anagawa-4-chome, Inage-ku, 263-8555, Chiba, Japan. drhmmura@nirs.go.jp |
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Abstract: | Heavy ion radiation (high linear energy transfer, LET, radiation) induces various types of chromosome aberration. In this report, we describe a new method employing an atomic force microscope (AFM) for nanometer-level structural analysis of chromosome damage induced by heavy ion irradiation. Metaphase mouse chromosomes with chromatid gap or chromatid breaks induced by heavy ion irradiation were marked under a light microscope. Then the detailed structure of chromosomes of Giemsa-stained or unstained samples was visualized by the AFM. The height data of chromosomes obtained by AFM provided useful information to distinguish chromatid gaps and breaks. A fibrous structure was observed on the unstained chromosome, the average width of which was about 45.8 nm in the image of AFM. These structures were considered to be 30-nm fibers on the chromosome. The structure of the break point regions induced by neon- or carbon-ion irradiation was imaged by AFM. In some cases, the fibrous structure of break points was detected by AFM imaging after carbon ion irradiation. These observations indicated that AFM is a useful tool for analysis of chromosome aberrations induced by heavy ion radiation. |
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