A preparation and purification of [1-14C]acetylcarnitine. |
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| Authors: | G Roughan J Browse |
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| Affiliation: | Horticulture Research Institute, Auckland, New Zealand. |
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| Abstract: | [1-14C]Acetylcarnitine was prepared from [1-14C]acetate and L-carnitine using acetyl-CoA synthetase and carnitine acetyltransferase. The product was purified by ion-exchange and thin-layer chromatography. Conversion of [1-14C]acetate to [1-14C]acetylcarnitine was better than 90% and overall recovery of the pure product was greater that 80%. |
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