毕赤酵母优化表达外源蛋白策略

毕赤酵母(Pichia pastoris)表达系统是一种异源蛋白表达的理想系统,但目前并非所有的外源蛋白都能在毕赤酵母中成功高效表达,不同的蛋白表现为不同表达水平、生物活性及稳定性。从遗传因素和表达条件综述了外源蛋白在毕赤酵母中的优化表达策略。

Strategies for Optimization Expression of Heterologous Protein in Pichia pastoris

Pichia pastoris is easy to genetically manipulate and can be grown to high cell densities, at the same time, P. pastoris is a eukaryote, and thereby has the potential for producing soluble, correctly folded recombinant protein with high yield, so the P. pastoris expression system is an ideal choice for the production of various heterologous proteins. However, at present not all the heterologous proteins can be successfully and efficiently expressed in P. pastoris, as a result, different proteins present different yield levels, bioactivity and stability. Here strategies for reducing proteolytic degradation and improving production of the expressed heterologous proteins are briefly summarized in terms of genetically factors and cultivation level.