Characterization of proton production and consumption associated with microbial metabolism |
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| Authors: | Karthikeyan Srinivasan Radhakrishnan Mahadevan |
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| Affiliation: | (1) Department of Chemical Engineering and Applied Chemistry, University of Toronto, M5S3E5 Toronto, Ontario, Canada;(2) Institute of Biomaterials and Biomedical Engineering, University of Toronto, M5S3G9 Ontario, Canada |
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| Abstract: | Background Production or consumption of protons in growth medium during microbial metabolism plays an important role in determining the pH of the environment. Such pH changes resulting from microbial metabolism may influence the geochemical speciation of many elements in subsurface environments. Protons produced or consumed during microbial growth were measured by determining the amount of acid or base added in a 5 L batch bioreactor equipped with pH control for different species including Escherichia coli, Geobacter sulfurreducens, and Geobacter metallireducens. |
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