Biochemical and molecular characterization of a rhizobitoxine-producing <Emphasis Type="Italic">Bradyrhizobium</Emphasis> from pigeon pea plants |
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Authors: | Kanika Tripti Dogra Lata |
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Institution: | (1) College of Science, King Saud University, Riyadh, SA, Saudi Arabia;(2) Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202002, U.P., India |
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Abstract: | Out of a total of 8 bacterial strains isolated from the root nodules of pigeon pea plants grown in arid region, five were
identified as rhizobia based on biochemical test and confirmed by 16S rDNA sequencing. PCR based screening for the rtxA gene (involved in biosynthesis of rhizobitoxine) revealed that the gene was present in one strain identified biochemically
and genetically as belonging to species Bradyrhizobium (BS KT-24). The strain was resistant to phosphomycin, nalidixic acid, kanamycin, gentamicin and neomycin but sensitive towards
streptomycin and spectinomycin. Bioinformatic-tool-guided phylogenetic analysis of rtxA gene revealed its distinctiveness from other known rtxA genes (present in B. japonicum, B. elkanii and Xanthomonas oryzae). The rhizobitoxine producing strain BS KT-24 is considered to exhibit better survival and nodulation protection besides
competitiveness for pigeon pea and other legumes grown under abiotic stress and, thus, be a candidate in practical aspect
of rhizobitoxine production by rhizobium and its application as rhizobial inoculants. |
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