Effect of Indole-3-Acetic Acid (IAA) Produced by Pseudomonas aeruginosa in Suppression of Charcoal Rot Disease of Chickpea |
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Authors: | Ekta Khare Naveen Kumar Arora |
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Institution: | (1) Department of Microbiology, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208024, UP, India; |
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Abstract: | The production of indole-3-acetic acid (IAA), by rhizobacteria, has been associated with plant growth promotion, especially
root initiation and elongation. Isolate TO3 selected from 103 fluorescent pseudomonads, identified as Pseudomonas aeruginosa, showed maximum production of IAA. Isolate TO3 having biocontrol activity against Macrophomina phaseolina also showed production of siderophore and HCN was used to screen the role of bacterial IAA in reducing the level of charcoal
rot disease occurrence in chickpea. Four IAA defective stable mutants of isolate TO3 having biocontrol activity against M. phaseolina were developed through 5-bromouracil mutagenesis. Mutant TO52 showed 76.47% reduction in production of IAA. Standard IAA was used in similar concentration as present in cell-free culture
supernatant of wild isolate TO3 and its mutant TO52. The in vitro and in vivo study showed that IAA-defective mutant TO52 caused reduced biocontrol and plant growth promotory activity than wild isolate TO3. Standard IAA showed comparable biocontrol
activity to the culture supernatant. To some extent better biocontrol and growth promotory activity in supernatant than standard
IAA indicates the synergistic role of siderophore and HCN. The study clearly reports the role of bacterial IAA in suppression
of charcoal rot disease of chickpea. |
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