人尿激酶原乳腺定位转基因小鼠的建立

应用大鼠β乳酪蛋白基因的上游调控序列和人尿激酶原ｃＤＮＡ构建成功了乳腺定位表达载体．用显微注射的手段导入到受精卵的雄前核，从注射的３００枚受精卵中，１４０枚被移植到９只假孕的受体小鼠．结果从获得的子一代小鼠中，经ＰＣＲ和Ｓｏｕｔｈｅｒｎｂｌｏｔ证实，有３只转基因阳性的小鼠．

Construction of Mammary Gland Specific Transgenic Mice of Human Prourokinase

The mammary gland specific expression vector of human prourokinase(Pro UK) was constructed using rat β casein upstream regulatory sequences and Pro UK cDNA as well as SV40 early polyA.The fusion genes were microinjected into fertilized eggs of mice.All positive transgenic mice were identified by PCR and Southern blot analysis of tail DNA with a random prime labeled fragment amplified from the fusion genes of rat β casein and Pro UK cDNA.Of the 300 eggs injected with the vector,140 were transferred to 9 recipient pseudopregnant mice,and 3 of the 15 offspring contained the transgene.