Primary culture of antvenom gland cells |
| |
Authors: | W F Hink J J Butz |
| |
Institution: | (1) Department of Entomology, Ohio State University, 43210 Columbus, Ohio |
| |
Abstract: | Summary Venom from the antPseudomyrmex triplarinus reduces the symptoms and swelling of rheumatoid arthritis. The cells that produce the venom were dissected from larval and
pupal ants and culture conditions studied. Cell dissociation, with minimal amount of damage, was done with 0.25% trypsin at
4°C with subsequent use of soybean trypsin inhibitor. A new medium was formulated and epidermal growth factor, fibroblast
growth factor, insulin, cAMP, cGMP, and isoproterenol were beneficial. The optimum osmotic pressure was a relatively high
500 mOsm. Conditioning the medium with an established insect cell line was essential for long-term cell survival. Under these culture
conditions the structural and metabolic integrity of the cells were maintained for up 12 mo. |
| |
Keywords: | venom gland culture antiarthritic factor |
本文献已被 SpringerLink 等数据库收录! |
|