Unusual increase of Scd1 and Elovl6 expression in rat inguinal adipose tissue |
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Authors: | Jacek Turyn Adriana Mika Piotr Stepnowski Julian Swierczynski |
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Institution: | 1.Department of Biochemistry,Medical University of Gdańsk,Gdańsk,Poland;2.Faculty of Biology,University of Gdańsk,Gdańsk,Poland;3.Institute for Environmental and Human Health Protection,University of Gdańsk,Gdańsk,Poland |
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Abstract: | It is generally accepted that the location of body fat deposits may play an important role in the risk of developing some
endocrine and metabolic diseases. We have studied the effect of food restriction and food restriction/refeeding, often practiced
by individuals trying to lose body weight, on the expression of genes which are associated with obesity and certain metabolic
disorders in inguinal, epididymal, and perirenal rat white adipose tissues. Gene expression was analyzed by real time semi-quantitative
polymerase chain reaction and by Western blot. We found that prolonged food restriction caused a significant decrease of body
and adipose tissue mass as well as the increase of Scd1 and Elovl6 gene expressions in all main rat adipose tissue deposits.
Food restriction/refeeding caused increases of: a) Scd1 and Elovl6 mRNA levels in adipose tissue, b) Scd1 protein level and
c) desaturation index in adipose tissue. The increased expression of both genes was unusually high in inguinal adipose tissue.
The results suggest that the increase of Scd1 and Elovl6 gene expressions in white adipose tissue by prolonged food restriction
and prolonged food restriction/refeeding may contribute to accelerated fat recovery that often occurs in individuals after
food restriction/refeeding. |
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