Immobilization of alcohol dehydrogenase by gel entrapment of cells of Saccharomyces cerevisiae |
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| Authors: | S.S. Godbole,S.F. D&#x Souza,G.B. Nadkarni |
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| Affiliation: | Biochemistry and Food Technology Division, Bhabha Atomic Research Centre, Bombay 400 085, India |
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| Abstract: | A stable immobilized preparation of alcohol dehydrogenase (ADH) (EC 1.1.1.1) was obtained by entrapment of ADH-containing Saccharomyces cerevisiae cells in polyacrylamide, polymerized by gamma-rays (100 kR). The permeability barrier for the substrate through the cell membrane was found to be eliminated on entrapment. The stability characteristics, pH-activity profile and other properties of the entrapped ADH are presented. A four-fold enhancement in Km for NAD+ was observed on entrapment, whereas Km for ethanol was not altered. |
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