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Inheritance and linkage relationships of isozyme loci in cucumber (Cucumis sativus L.)
Authors:L. D. Knerr  J. E. Staub
Affiliation:(1) Department of Horticulture, University of Wisconsin, 53706 Madison, WI, USA;(2) Vegetable Crops Research, USDA/ARS, Department of Horticulture, University of Wisconsin, 53706 Madison, WI, USA;(3) Present address: Ferry-Morse Seed Co, 1570 County Rd. 951, N., 33999 Naples, FL, USA
Abstract:Summary Genetic analyses were conducted among 18 provisionary isozyme loci in Cucumis sativus L. Fourteen loci demonstrated simple Mendelian inheritance while observed variation at four loci (Gpi2, Gr2, Pgm3, Skdh2) was determined not to have a predictable genetic basis. Joint segregation analyses among the 14 genetically predictable polymorphic loci resulted in the assignment of 12 loci to four linkage groups. Linkage groups contain the following loci: (1) Gr1, Pgm1, Idh, Pgd1; (2) Pep-pap, Mdh2, Mdh3, Gpi1; (3) Pep-la, Per4; (4) Pgd2, G2dh. Mpi2 and Mdh1 segregated independently. Recombination fractions for linked loci ranged between 0.051 (Pgm1-Idh) to 0.385 (Pep-la-Per4). Some practical applications of isozyme marker loci for cucumer improvement are discussed.
Keywords:Cucumber  Gene mapping  Isozymes  Segregation  Starch-gel electrophoresis
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