Improving genetic transformation of European chestnut and cryopreservation of transgenic lines |
| |
Authors: | E Corredoira M C San-José A M Vieitez A Ballester |
| |
Institution: | (1) Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Avenida de Vigo, s/n, Apartado 122, Santiago de Compostela, 15080, Spain |
| |
Abstract: | The aim of the present work was to study the effect of the developmental stage of the somatic embryos and of the genotype
on the genetic transformation of embryogenic lines of European chestnut (Castanea sativa Mill.) and the cryopreservation of the embryogenic lines that are generated. As an initial source of explants in the transformation
experiments, it was found that the use of somatic embryos isolated in the globular stage or clumps of 2–3 embryos in globular/heart-shaped
stages was more effective (30%) than when embryos at the cotyledonary stage were used (6.7%). All of the seven genotypes tested
were transformed, and transformation efficiency was clearly genotype dependent. Three transgenic lines were successfully cryopreserved
using the vitrification procedure, and the stable integration of the uidA gene into the transgenic chestnut plants that were regenerated subsequent to cryopreservation was demonstrated. |
| |
Keywords: | Agrobacterium Castanea sativa Embryogenic lines |
本文献已被 SpringerLink 等数据库收录! |
|