13C NMR cross polarization and magic angle spinning (CPMAS) and gas chromatography/mass spectrometry analysis of the products from a soda pulp mill effluent decolourised by two Streptomyces strains |
| |
Authors: | M Hernández J Rodríguez M I Pérez A S Ball M E Arias |
| |
Institution: | (1) Departamento de Microbiología y Parasitología, Universidad de Alcalá de Henares, 28871 Alcalá de Henares, Madrid, Spain Fax: +341 8854623 e. mail: MPMAF@ALCALA.ES, ES;(2) Department of Biological and Chemical Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, England, GB |
| |
Abstract: | Two Streptomyces strains, UAH 30 and UAH 51, have been shown to decolourise a paper-mill effluent obtained after semichemical alkaline pulping
of wheat straw. Fractionation of the effluent decolourised by strains UAH 30 and UAH 51 showed that 60% and 80% respectively
of the alkali-lignin fraction have been removed from the effluent after 7 days of growth. 13C NMR cross polarization and magic angle spinning (CPMAS) spectra of the alkali-lignin remaining in the effluent after decolourisation
revealed a decrease in the relative amount of aromatic lignin units compared to that obtained from the untreated effluent
along with a reduction in the ratio of syringyl:guaiacyl units. Gas chromatography/mass spectrometry analysis of the low-molecular-mass
compounds extracted from the decolourised effluent revealed the presence of new aromatic lignin-related compounds that were
not present in the untreated control effluent. This was linked to a general depolymerization of larger lignin molecules occurring
during decolourisation by the two Streptomyces strains. Identification of low-molecular-mass aromatic compounds extracted from the decolourised effluent revealed only the
presence of p-hydroxyphenyl units in effluents decolourised by the strain UAH 30 while p-hydroxyphenyl, guaiacyl and syringyl units were detected in effluents decolourised by Streptomyces strain UAH 51. The study indicates that, while decolourisation is a common feature of the two Streptomyces strains, the mechanisms involved in the degradation of the lignin fractions may be different and strain-specific.
Received: 8 July 1996 / Received revision: 9 October 1996 / Accepted: 14 October 1996 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|