Identification of the gene encoding DNA topoisomerase I from Candida albicans |
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Authors: | Alison Taylor Kellie Giles Aparna V Sarthy Tom McGonigal Jennifer Fostel |
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Institution: | Antifungal Biology, Anti-infective Division, Abbott Laboratories D-47M Building AP9A, 100 Abbott Park Rd.,Abbott Park, IL 60064, USA |
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Abstract: | Abstract A gene encoding a type I topoisomerase (TOP1) was isolated from Candida albicans , sequenced, and expressed in Saccharomyces cerevisiae . The TOP1 gene was identified from a C. albicans genomic library by hybridization with the product of a polymerase chain reaction with degenerate primer sets encoding regions conserved in other TOP1 genes. A clone containing an open reading frame of 2463 bp and predicted to encode a protein of 778 amino acids with sequence similarity to eukaryotic type I topoisomerases was identified. The C. albicans TOP1 gene restored camptothecin sensitivity and increased the topoisomerase activity in S. cerevisiae , indicating that the DNA fragment encodes a functional C. albicans topoisomerase I. |
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Keywords: | Antifungal target Camptothecin Candida TOP01 DNA sequence Topoisomerase I |
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