Expression and characterization of the 42 kDa chitinase of the biocontrol fungus Metarhizium anisopliae in Escherichia coli |
| |
Authors: | Baratto César Milton da Silva Marcia Vanusa Santi Lucélia Passaglia Luciane Schrank Irene Silveira Vainstein Marilene Henning Schrank Augusto |
| |
Affiliation: | Centro de Biotechnologia, Universidade Federal do Rio Grande do Sul, UFRGS, Porto Alegre, RS, Brazil. |
| |
Abstract: | Albeit Metarhizium anisopliae is the best-characterized entomopathogenic fungus, the role of some hydrolytic enzymes during host cuticle penetration has not yet been established. Three chitinase genes (chit1, chi2, chi3) from Metarhizium have already been isolated. To characterize the chitinase coded by the chit1 gene, we expressed the active protein (CHIT42) in Escherichia coli using a T7-based promoter expression vector. The recombinant protein, CHIT42, is active against glycol chitin and synthetic N-acetylglucosamine (GlcNAc) dimer and tetramer substrates. These activities suggest that the recombinant CHIT42 acts as an endochitinase. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|