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Direct measurement of proton binding to the active ternary complex of pig heart lactate dehydrogenase (Short Communication) |
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| Authors: | J John Holbrook |
| Institution: | Department of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K. |
| Abstract: | With the use of a glass electrode it is shown that one proton is taken up from the solvent when o-nitrophenylpyruvate forms a ternary complex with lactate dehydrogenase and NADH at pH8 and before the complex has rearranged to give products. |
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