Callus and embryoid formation from protoplasts of Helianthus annuus |
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Authors: | Anne-Laure Moyne Véronique Thor Bernard Pelissier Catherine Bergounioux Georges Freyssinet Pierre Gadal |
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Institution: | (1) Laboratoire de Physiologie végétale moléculaire (UA CNRS 1128), bâtiment 430, Université Paris-Sud, 91405 Orsay, Cedex, France;(2) Rhone-Poulenc Agrochimie, 14-20 rue Pierre-Baizet, BP 9163, 69263 Lyon Cedex 09, France |
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Abstract: | Sunflower hypocotyl protoplasts have been isolated and cultured. Optimum plating density for cell division and colony formation was in the range of 5 to 7×104 cells/mi in an agarose medium supplemented with BAP (1 mg/l) and NAA (1 mg/l). Plating efficiency was 60% after 21 days of culture. In the resultant culture a mixed population of calli and embryoids was observed. Thirty seven percent of the cell clusters exhibited a developmental pattern similar to an embryoid. Many stages of embryogenesis were observed in the same cultures.Abbreviations 2,4-D
2,4 dichlorophenoxyacetic acid
- NAA
1-naphtaleneacetic acid
- IAA
Indole-3-acetic
- BAP
6-benzylamino purine
- GA3
Gibberellic acid |
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Keywords: | Protoplasts Helianthus annuus Somatic embryogenesis Callus formation |
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