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1株抑制土传病原菌且产纤维素酶芽胞杆菌筛选及产酶条件的研究
引用本文:刘晓辉,陈顺,陈飞,王红旗,桓明辉,高晓梅,张岩,于广峰,孙立梅,郭玲玲.1株抑制土传病原菌且产纤维素酶芽胞杆菌筛选及产酶条件的研究[J].微生物学杂志,2011,31(1):63-67.
作者姓名:刘晓辉  陈顺  陈飞  王红旗  桓明辉  高晓梅  张岩  于广峰  孙立梅  郭玲玲
作者单位:辽宁省微生物科学研究院,辽宁,朝阳,122000
基金项目:国家农业科技成果转化项目,辽宁省科技厅攻关计划项目
摘    要:通过刚果红染色法和DNS分光光度法对6种芽胞杆菌分泌胞外纤维素酶进行筛选,再通过管碟法测试对5种病原菌的抑菌作用,得到1株芽胞杆菌(菌株编号为X-02)酶活达182.5 U/mL,而且对几种土传病害有抑制作用。并对其产酶发酵培养基碳源、氮源及初始pH、发酵温度、接种量、摇瓶转速和时间进行优化,结果显示该菌株最佳碳源是2%CMC-Na,其次是葡萄糖,二者产酶之差只为21 U/mL。考虑大量生产的成本和方便性(CMC-Na溶解慢),选择葡萄糖为碳源,氮源为2.0%蛋白胨与酵母膏复合氮源,在pH值为8.0、温度37℃、接种量为2%、转速为180 r/m in、时间48 h条件下酶活达到391.0 U/mL酶液,比优化前提高了2.1倍。

关 键 词:纤维素酶  芽胞杆菌  发酵  管碟法  病原菌

Screening and Enzyme Producing Conditions of Bacillus Strain Inhibited against Soil Borne Pathogen and Produced Cellulase
LIU Xiao-hui,CHEN Shun,CHEN Fei,WANG Hong-qi,HUAN Ming-hui,GAO Xiao-mei,ZHANG Yan,YU Guang-feng,SUN Li-mei,GUO Ling-ling.Screening and Enzyme Producing Conditions of Bacillus Strain Inhibited against Soil Borne Pathogen and Produced Cellulase[J].Journal of Microbiology,2011,31(1):63-67.
Authors:LIU Xiao-hui  CHEN Shun  CHEN Fei  WANG Hong-qi  HUAN Ming-hui  GAO Xiao-mei  ZHANG Yan  YU Guang-feng  SUN Li-mei  GUO Ling-ling
Institution:LIU Xiao-hui,CHEN Shun,CHEN Fei,WANG Hong-qi,HUAN Ming-hui,GAO Xiao-mei,ZHANG Yan,YU Guang-feng,SUN Li-mei,GUO Ling-ling(Liaoning Acad.of Microbiol.Sci.,Chaoyang 122000)
Abstract:A Bacillus strain(serial number X-02) was screened among six kinds of Bacillus that excrete extracellular cellulase were screened through DNS spectrophotometer and the Congo red staining,by testing with 5 kinds of pathogen for their inhibition with cup-plate method;and the enzyme activity was as high as 182.5 U/mL and had the inhibition against several soil borne pathogens.Later carbon source,nitrogen source and initial pH of its fermentation medium,temperature,inoculation quantity,rotation of shake flask a...
Keywords:cellulase  Bacillus  fermentation  cup-plate method  pathogen  
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