Identification of RAPD markers linked to a major blast resistance gene in rice |
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| Authors: | Naweed I. Naqvi J. Michael Bonman David J. Mackill Rebecca J. Nelson Bharat B. Chattoo |
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| Affiliation: | (1) Dept. of Microbiology and Biotechnology Center, Faculty of Science, Maharaja Sayajirao University, 390 002 Baroda, India;(2) Du Pont Agricultural Products, P.O. Box 30, 19714 Newark, DE, USA;(3) USDA-ARS, Dept. of Agronomy & Range Science, University of California, 95616 Davis, CA, USA;(4) International Rice Research Institute, P.O. Box 933, 1099 Manila, Philippines |
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| Abstract: | Rice blast, caused byPyricularia grisea, is a major production constraint in many parts of the world. The Korean rice variety Tongil showed high levels of resistance for about six years when widely planted under highly disease-conducive conditions, before becoming susceptible. Tongil was found to carry a single dominant gene, designatedPi-10t, conferring resistance to isolate 106 of the blast pathogen from the Philippines. We report here the use of bulked segregant RAPD analysis for rapid identification of DNA markers linked toPi-10t. Pooled DNA extracts from five homozygous blast-resistant (RR) and five susceptible (rr) BC3F2 plants, derived from a CO39 × Tongil cross, were analyzed by RFLP using 83 polymorphic probes and by RAPD using 468 random oligomers. We identified two RAPD markers linked to thePi-10t locus: RRF6 (3.8 ± 1.2 cM) and RRH18 (2.9 ± 0.9 cM). Linkage of these markers withPi-10t was verified using an F2 population segregating forPi-10t. The two linked RAPD markers mapped 7 cM apart on chromosome 5. Chromosomal regions surrounding thePi-10t gene were examined with additional RFLP markers to define the segment introgressed from the donor genome.Pi-10t is likely to be a new blast-resistance locus, because no other known resistance gene has been mapped on chromosome 5. These tightly linked RAPD markers could facilitate early selection of thePi-10t locus in rice breeding programmes. |
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| Keywords: | Gene mapping near-isogenic lines Oryza sativa l. Pyricularia grisea Cav. Pyricularia oryzae Cav. |
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