Diversity of genetic systems responsible for naphthalene biodegradation in <Emphasis Type="Italic">Pseudomonas fluorescens</Emphasis> strains |
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| Authors: | Email author" target="_blank">T?Yu?IzmalkovaEmail author O?I?Sazonova S?L?Sokolov I?A?Kosheleva A?M?Boronin |
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| Institution: | (1) Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, 142290 Pushchino, Moscow oblast, Russia;(2) Pushchino State University, pr. Nauki 3, 142290 Pushchino, Moscow oblast, Russia |
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| Abstract: | The genetic systems that are responsible for naphthalene catabolism were analyzed in 18 naphthalene-degrading Pseudomonas fluorescens strains isolated from oil-contaminated soils in different regions of Russia. It was found that 13 strains contain plasmids, from 20 to 120 kb in size, at least 5 of which are conjugative and bear the catabolic genes responsible for the complete utilization of naphthalene and salicylate. Five plasmids belong to the P-7 incompatibility group, and two plasmids belong to the P-9 incompatibility group. The naphthalene biodegradation genes of P. fluorescens are highly homologous to each other. The study revealed a new group of the nahAc genes and two new variants of the nahG gene. The suggestion is made that the key genes of naphthalene biodegradation, nahAc and nahG, evolve independently and occur in P. fluorescens strains in different combinations.Translated from Mikrobiologiya, Vol. 74, No. 1, 2005, pp. 70–78.Original Russian Text Copyright © 2005 by Izmalkova, Sazonova, Sokolov, Kosheleva, Boronin. |
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| Keywords: | Pseudomonas fluorescens diversity naphthalene catabolism genes plasmids polymerase chain reaction |
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