The use of FRET imaging microscopy to detect protein-protein interactions and protein conformational changes in vivo. |
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Authors: | K Truong M Ikura |
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Institution: | Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, Canada. |
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Abstract: | Intermolecular and intramolecular FRET between two spectrally overlapping green fluorescent protein variants fused to two different host proteins or at two different sites within the same protein offers a unique opportunity to monitor real-time protein-protein interactions or protein conformational changes. By using fluorescence digital imaging microscopy, one can visualize the location of green fluorescent proteins within a living cell and follow the time course of the changes in FRET corresponding to cellular events at a millisecond time resolution. The observation of such dynamic molecular events in vivo provides vital insight into the action of biological molecules. |
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