Synthesis and reactivity of guanosine 3′,5′-phosphoric acid alkyl esters |
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Authors: | Joachim Engels |
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Institution: | Fachbereich Chemie, Universität Konstanz, Postfach 7733, D-7750 Konstanz, West Germany |
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Abstract: | Guanosine 3′,5′-phosphoric acid alkyl esters were synthesized by reaction of the free acid of guanosine 3′,5′-phosphate (cGMP) with the appropriate diazoalkane. The resulting pair of diastereoisomers was separated chromatographically, and the conformations were assigned on the basis of 31P-nmr. Hydrolysis of the compounds in water was measured, and their half-lives were determined. The ability of the compounds to act as substrates for phosphodiesterase from beef heart was tested, and their inhibition of this enzyme was measured. |
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