Synthesis of [Gln4]-neurotensin using a new solid support,the 4-(hydroxymethyl)phenylacetamidomethyl-resin

For biomedical studies, [Gln⁴]-neurotensin, which appears to be the naturally occurring form of neurotensin, was synthesized using a recently designed new resin for the Merrifield solid-phase synthesis of peptides, 4-(hydroxymethyl)phenylacetamidomethyl resin (PAM-resin). This synthesis was compared to the synthesis of [Gln⁴]-neurotensin by the use of oxymethyl-copoly-(styrene-divinylbenzene) as the solid support. The PAM-resin was superior, since the yield of [Gln⁴]-neurotensin was doubled and fewer purification steps were necessary.