A newly synthesized protein interacts with GroES on the surface of chaperonin GroEL. |
| |
Authors: | E S Bochkareva A S Girshovich |
| |
Affiliation: | Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel. |
| |
Abstract: | To facilitate folding and assembly of different proteins, chaperonin GroEL requires the presence of its helper protein GroES. Using a photochemical cross-linking approach, we show that GroES and newly synthesized pre-beta-lactamase (pre-beta lac) contact with each other only within the ternary complex with GroEL. Possibly owing to this contact GroES is able to directly influence the pre-beta lac/GroEL interaction. Furthermore, the cross-linking of pre-beta lac to GroES suggests that the binding of the protein ligands to GroEL occurs near the GroES binding site, known to be in the central hole space of GroEL. |
| |
Keywords: | |
|
|