顺乌头酸酶基因在杀雄剂SQ-1诱导小麦雄性不育花药中的表达分析

采用RT-PCR技术,克隆了小麦胞质顺乌头酸酶基因(cACO)部分cDNA序列.该cDNA序列长1368bp,编码456个氨基酸,GenBank登录号为GU475062.半定量RT-PCR结果表明,在生理型不育和可育花药发育的单核早期至三核期,cACO基因的表达水平均表现为先升后降;在生理型不育花药发育的单核晚期cACO基因表达水平与同期可育花药相比显著升高,到二核期和三核期明显降低,ACO酶活性变化表现出相同趋势.这反映出在小麦生理型不育系中,cACO基因在花药败育关键期异常表达可能影响了花药发育过程中正常的能量供应和物质代谢,导致花粉发育能量不足和所需物质匮乏,从而导致了非遗传型花药败育现象.

Expressing Analysis of cACO Gene in Male Sterile Wheat Induced by Chemical Hybridizing Agent SQ-1

A cDNA sequence partially encoding cytosolic aconitase (cACO) was cloned by RT-PCR. The cloned cDNA sequence was 1 368 bp and putatively encoded a peptide of 456 amino acids, and the assigned GenBank accession was GU475062. The expression pattern of cACO gene between sterile and fertile anthers was analyzed by semi-quantitative RT-PCR. The results showed that the expression levels of cACO gene in sterile and fertile anthers were firstly upregulated and then decreased, but the expression level of cACO gene in sterile anthers was significantly higher than that in fertile anthers at the late mononucleate stage, and then its expression level was much lower than that in fertile anthers at binucleate and trinucleate stages. The dynamic change of assayed cACO activity was consistent with the results proven by semi-quantitative RT-PCR. Thus the shortage of energy and some metabolic intermediates caused by abnormal expression of cACO gene probably contributed to the abortion of pollens at the key developmental stage in chemical hybrid agents SQ-1 induced male sterility in wheat.