Alteration in growth and peroxidase activity by heavy metals in Phaseolus seedlings |
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Authors: | N G Parmar S D Vithalani S V Chanda |
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Institution: | (1) Department of Biosciences, Saurashtra University, 360 005 Rajkot, India |
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Abstract: | The aim of the present work was to see the effect of mercury and chromium on elongation growth of phaseolus seedlings and
changes in chlorophyll content. Phaseolus seedlings were treated with two different concentrations of two heavy metals viz. mercury (0.05 mM and 0.4 mM HgCl2, and chromium (0.5 mM and 1.0 mM K2Cr2O7). Both mercury and chromium inhibited root and hypocotyl elongation growth. Changes in cytoplasmic and wall bound peroxidase
activities were studied using guaiacol as a hydrogen donor. Peroxidase activity was higher in both mercury and chromium treated
seedlings as compared to distilled water control; they showed a clear concentration effect. Peroxidase activity showed inverse
relation with growth i.e. distilled water treated seedlings had maximum growth and minimum activity while higher concentration of heavy metal treated
seedlings had minimum growth and maximum activity. Chlorophyll content was also decreased by mercury. The role of peroxidase
activity in defense mechanism in response to heavy metal toxicity is discussed. |
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Keywords: | Phaseolus vulgaris mercury chromium metal toxicity peroxidase activity |
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