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| 重楼属植物DNA提取的方法研究 | |
| 引用本文： | 张金渝,文国松,虞泓,丁长春. 重楼属植物DNA提取的方法研究[J]. 生物技术, 2005, 15(4): 39-41 |
| 作者姓名： | 张金渝 文国松 虞泓 丁长春 |
| 作者单位： | 1. 云南大学生命科学院生态遗传学实验室,云南,昆明,650091;云南省农业科学院药用植物研究所,云南,昆明,650205 2. 云南农业大学生物技术学院,云南,昆明,650204 3. 云南大学生命科学院生态遗传学实验室,云南,昆明,650091;云南英茂生物技术实验室,云南,昆明,650212 4. 云南大学生命科学院生态遗传学实验室,云南,昆明,650091 |
| 基金项目： | 云南大学省级生物技术人才培养基地资助项目 |
| 摘 要： | 为了获得重楼属植物高质量的DNA，以研磨、冻融和细胞溶胀破膜的原理设计了一种DNA提取的方法并与CTAB法和高盐法比较。结果显示该方法提取的重楼属植物DNA纯度（A260/A280=1.792-1.852;A260/A230=2.052-2.267）,产率（6.12-12.84mg/g）较其他两种方法高，降解少，节省药品。所提出的DNA能用于RAPD分析和ITS测序。 |
| 关 键 词： | 重楼属植物 DNA提取 |
| 文章编号： | 1004-311X（2005）04-0039-03 |
| 收稿时间： | 2004-11-12 |
| 修稿时间： | 2005-04-11 |
Study on Extraction Methods of Paris Genomic DNA |
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| ZHANG Jin-Yu,WEN Guo-song,YU Hong,DING Chang-chun. Study on Extraction Methods of Paris Genomic DNA[J]. Biotechnology, 2005, 15(4): 39-41 | |
| Authors: | ZHANG Jin-Yu WEN Guo-song YU Hong DING Chang-chun |
| Abstract: | Basing on principles of grind, freezing and thawing, cell dissolution, a kind of method was devised for high quality DNA extraction of Paris, and the results of 3 kinds of method for extracting DNA were compared using Paris as materials. The results show that the purity (A_260 /A_280 =1.792-1.852; A_260 /A_ 230 =2.052-2.267) and yield (6.12-12.84mg/g) of extracted DNA by our method was higher than tow other methods, the kind of DNA extraction method was economical. Extracted DNA by our method was integrated , and suitable for RAPD analysis and ITS sequencing. |
| Keywords: | Paris DNA extraction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |